Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human Fluids

SERS_Quantification_of_StaphylococcusOnline SERS quantification of Staphylococcus aurens

Carme Catala, Bernat Mir-Simon, Xiaotong Feng, Celia Cardozo, Nicolas Pazos-Perez, Elena Pazos, Sara Gómez-de Pedro, Luca Guerrini, Alex Soriano, Jordi Vila, Francec Marco, Eduardo Garcia-Rico and Ramon A. Alvarez-Puebla
Advanced Materials Technologies - DOI: 10.1002/admt.201600163

Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (~1 milliliter) extending over 24–72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (~1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.

SERS Surface Selection Rules for the Proteomic Liquid Biopsy in Real Samples: Efficient Detection of the Oncoprotein c-MYC

SERS surface selection rules for the proteomic liquid biopsy in real samples

E. Pazos; M. García-Algar; C. Penas; M. Nazarenus; A.Torruella; N. Pazos- Perez; L. Guerrini; M.E. Vázquez; E. García-Rico; J.L. Mascareñas; R.A. Alvarez-Puebla ;
Journal of the American Chemical Society, (2016) (DOI:10.1021/jacs.6b08957)

Blood-based biomarkers (liquid biopsy) offer extremely valua-ble tools for the non-invasive diagnosis and monitoring of tumors. The protein c-MYC, a transcription factor that has been shown to be deregulated in up to 70% of human cancers, can be used as a robust proteomic signature for cancer. Herein, we developed a rapid, highly specific and sensitive SERS assay for the quantification of c-MYC in real blood samples. The sensing scheme relies on the use of specifically designed hybrid plasmonic materials and their bioderivatization with a selective peptidic receptor modified with a SERS transducer. Peptide/c-MYC recognition events translate into measurable alterations of the SERS spectra associated with a molecular re-orientation of the transducer, in agreement with the surface selection rules. The efficiency of the sensor is demonstrated in cellular lines, healthy donors and a cancer patient.




Energy Solutions Inspired by Nature

Energy Solutions Inspired by Nature

Adrianna Nogalska, Kamila Szalata, Tania Gumi, Jose Antonio Reina, Marta Giamberini, Bartosz Tylkowski, Ricard Garcia Valls
Energy Today - official e-magazin of America Energy Association

During the last decade society demanded to energy providers solutions on production and storage of alternative/green fuels. The market calls for new upstream technological innovations to respond sustainably, efficiently and safely to current and future energy needs. For this reason, most of companies decided to dedicate their R&D departments to find achievable solutions to these societal demands.


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